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Tynax ~ Patent Library

Technology for License:

A Rapid Diagnostic Kit and Reagents for Detecting Dengue Infections    

The kit relates to diagnosis of the four known closely related, antigenically distinct serotypes of Dengue virus.

Overview

The technology relates to novel kit and reagents for diagnosis of Dengue viral infections. The kit relates to diagnosis of the four known closely related, antigenically distinct serotypes of Dengue virus. More particularly, the technology relates to multiepitope recombinant proteins and their use in the diagnosis of dengue and other viral infections. The technology provides two dengue multiepitope proteins, one designed to detect IgM (Immunoglobin M) and the other to detect IgG (Immunoglobin G) antibodies in dengue patient sera. An important aspect of the technology resides in designing and expressing two novel recombinant protein antigens by assembling key immunodominant linear dengue-specific epitopes, chosen on the basis of pepscan analysis, phage display and computer predictions. One of these developed to specifically detect dengue-specific IgM and the other to detect IgG These novel recombinant dengue multiepitope proteins were expressed in E. coli, purified in a single step and used as capture antigens in ELISA. The ELISA results, using a large panel of suspected dengue patient sera, were in excellent agreement with those obtained using the commercially available Dengue Duo IgM and IgG Rapid strip test (PanBio). The technology also provides a simple and rapid spot test for dengue detection using these recombinant multiepitope proteins. The high epitope density, careful choice of epitopes and the use of E. coli system for expression has resulted in inexpensive diagnostic tests kits and methods with a high degree of sensitivity and specificity.

The Problem Solved by the Technology

Need to develop inexpensive, simple and rapid diagnostic tests, without compromising on sensitivity and specificity.

How the Technology Solves the Problem

A novel kit and reagents for diagnosis of Dengue viral infections. The kit relates to diagnosis of the four known closely related, antigenically distinct serotypes of Dengue virus.

Primary Application of the Technology

Dengue detection

Competitive Advantage

Simple rapid spot test for dengue detection

Notes on Development Status

Proof of concept developed.

Comments on Deal Structure, Potential Terms and Restrictions

Patent applied for. Available for licensing; Buyer-side commission fee