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Patent for License:

Unique Model for Screening Anti-Diabetic Compounds    

The technology provides a method for generating insulin non-responsive skeletal muscle cell culture model to screen anti-diabetic compounds against insulin resistance.

Overview

The technology provides a technique for generating insulin non-responsive skeletal muscle cell culture model to screen anti-diabetic compounds against insulin resistance. The technique comprises:

a) growing myocytes in serum containing medium;

b) differentiating the myocytes into myotubes in a mixture of serum free medium in the continuous presence of insulin,

c) determining the insulin-stimulated tyrosine phosphorylation of Insulin Receptor (IR) and/or Insulin receptor substrate-1 (IRS-1) myotubes differentiated in chronic presence of insulin to that of control (same cells differentiated in a mixture of serum free medium in the absence of insulin), wherein the reduced stimulation of tyrosine phosphorylation of IR and/or IRS-1 in myotubes differentiated in continuous presence of insulin as compared to control, indicated the development of insulin resistance, and

d) screening the compounds to determine whether they are capable of enhancing insulin-simulated tyrosine phosphorylation of IR and/or IRS-1 myotubes differentiated in continuous presence of insulin to that of control (same cells are differentiated in the serum free medium in the absence of insulin).

Primary Application of the Technology

Animal-based research

The Problem Solved by the Technology

- Screening large number of compounds would be very expensive
- The variability among the animals makes it harder to compare the results and it also has the potential problems of animal ethics

How the Technology Solves the Problem

Skeletal muscle cell culture model

Competitive Advantage

The technology provides a technique for generating insulin non-responsive skeletal muscle cell culture model to screen anti-diabetic compounds against insulin resistance.

Comments on Deal Structure, Potential Terms and Restrictions

Available for licensing; Buyer-side commission fee

Patent Summary

U.S. Patent Classes & Classifications Covered in this listing:

Class 435: Chemistry: Molecular Biology And Microbiology

This class provides for the following subject matter when not provided for elsewhere: A process of using a microorganism or enzyme to synthesize a chemical product. A process of treating a material with a microorganism or enzyme to separate, liberate, or purify a preexisting substance. An in vitro process of measuring and testing in which: (1) A microorganism or enzyme is used to determine the presence or identity of a compound or composition in a sample; (2) A microorganism is identified by propagation; (3) An enzyme is identified by its catalytic activity; (4) The presence of microorganisms is detected; (5) A live microorganism is used in an antigen antibody test as an antigen; (6) Fixed or stabilized nonliving microorganisms, cells, or tissues are involved. A process of propagating a microorganism. A process in which the genetic structure of a microorganism or extrachromosomal genetic structure is altered. A process of organ or tissue maintenance. A process of mashing or malting. Microorganisms, per se, or the subcellular parts thereof. Enzymes, immobilized enzymes or enzyme containing compositions not otherwise provided for and the processes for purifying enzymes or forming immobilized enzymes. Compositions claimed or solely disclosed as for the propagation of microorganisms or for measuring and testing processes. Using microorganisms to destroy hazardous or toxic waste.

Subclass 325: ANIMAL CELL, PER SE (E.G., CELL LINES, ETC.); COMPOSITION THEREOF; PROCESS OF PROPAGATING, MAINTAINING OR PRESERVING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF ISOLATING OR SEPARATING AN ANIMAL CELL OR COMPOSITION THEREOF; PROCESS OF PREPARING A COMPOSITION CONTAINING AN ANIMAL CELL; CULTURE MEDIA THEREFORE
Subclass 354: Mouse (i.e., Mus)
Subclass 373: Method of co-culturing cells
Subclass 375: Method of regulating cell metabolism or physiology