Patent for License:

Nanopatch sensor in cell membrane    

A real-time novel cellular platform which may be used for detecting occurrence of cellular biochemical processes that involve membrane perturbation or take place at membrane surfaces

Overview

The technology provides a real-time novel cellular platform which may be used for detecting occurrence of cellular biochemical processes that involve membrane perturbation or take place at membrane surfaces. The cellular platform i.e a chemical construct (nanopatch) comprising polydiacetylenes and lipids may be assembled within the cell membrane of living cells, and that said construct retains its ability to undergo its characteristic chromatic (red to blue) transition and associated fluorescent emission in live cells in response to various perturbing agents and conditions. It has further been unexpectedly found that following insertion of said construct into the cell membrane of living cells, the viability of said cells is retained for a period of time that is sufficient to permit the use of said cells as a system for detecting and/or quantifying biochemical events that lead to structural and morphological changes in the cell membrane and/or other processes occurring at the surface of the cell membrane.

In contrast to existing cellular assays which, in general, detect specific chemical species or pathways within the cell, the cells and methods of the present invention are sensitive to any stimuli that induce functional and morphological modification of the cell membrane. A key feature of the claimed invention is the fact that following introduction of the nanopatches into the cell membrane, the target cells remain alive (and therefore useful for real-time investigations of processes in viable cells) for periods of at least four hours.

Primary Application of the Technology

Cellular assays, biological pathway studies, assay development and drug target identification and screening

The Problem Solved by the Technology

Real-time reporting of biological or chemical events that may affect the function and/or morphology and/or structural properties of either the cell membrane, or alternatively, the function, morphology and viability of the whole cell.

How the Technology Solves the Problem

May be used for detecting occurrence of cellular biochemical processes that involve membrane perturbation or take place at membrane surfaces. The cellular platform i.e a chemical construct (nanopatch) comprising polydiacetylenes and lipids may be assembled within the cell membrane of living cells, and that said construct retains its ability to undergo its characteristic chromatic (red to blue) transition and associated fluorescent emission in live cells in response to various perturbing agents and conditions.

Competitive Advantage

Real-time novel cellular platform , rapid screening of pharmaceuticals compounds in real-living cell scenarios.

Comments on Deal Structure, Potential Terms and Restrictions

Available for licensing; Buyer-side commission fee