Patent for Sale:

Healthy Food Method    

Genetic register and taxonomic identification of animal species in food samples - tool with diverse applications in the food industry

Overview

Diagnosing of unhealthy food products.

Detection of adulteration in food (chicken, beef, pork meat, etc.)

Guaranteeing of health species in food products. Avoiding presence of undesired specimens.

A genetic register and taxonomic identification system for the animal kingdom. This system has been conceived as a genetic and bioinformatic strategy based on a gene fragment common to all animal genomes which contains a high degree of evolutionary conservation. This approach enables the unification of experimental reagents irrespective of the biological origin of the sample to be tested, and allows for the handling and identification of processed or heterogeneous samples, i.e. those whose origin corresponds to more than one animal species. The genetic region under analysis is present in all forms of life and contains a genomic fragment which has varied its DNA content through evolution. The degree of sequence divergence accumulated by one species in this region becomes a sort of DNA barcode of that species, thus enabling its identification. The genetic register is a new taxonomic tool for the assessment and monitoring of biodiversity loss as well as a register of genetic biodiversity at the service of any activity that may require biological species identification. It is directly applicable to the control of animal biodiversity heritage for environmental protection agencies and companies or laboratories dealing with or supplying biological material.

Primary Application of the Technology

Diagnosing of unhealthy food products.
Detection of adulteration in food (chicken, beef, pork meat, etc.)
Through genetical and taxonomical identification of animal and plant species in food samples.

The Problem Solved by the Technology

• Molecular genetic techniques enable us to define the species’ origin of biological samples by looking at particular regions of the sample’s DNA, without the need to interrogate morphological features. A sort of DNA-barcode that identifies the species, and to some degree its evolutionary history. Some genomic regions have been recently described to show inter-species differences and can be useful for taxonomic purposes. These DNA-barcodes can only be used in pure or homogeneous biological material, i.e. that which originates only from one species or one individual animal. Experimental evaluation of these DNA-barcodes requires genetic reagents which are specific for that species or its taxonomic group. This is why currently available genetic species-identification methods require as many tests as species one wants to identify, which makes the process impossible to cover the more than 100,000 different species of living vertebrates alone.

How the Technology Solves the Problem

The genetic register eliminates this obstacle by the use of “universal” genetic reagents that can “read through” the same DNA fragment, highly conserved throughout evolution, and functional in the whole of the animal kingdom. This strategy also enables the identification of heterogeneous or processed biological samples, such as fish-fingers, mix-meat products, or animal remains found next to a natural reserve, without any previous knowledge or suspicion of its taxonomic classification. Furthermore, the genetic register provides the basis for the development of future applications for the monitoring of biodiversity fluctuations, due to natural (seasonal) effects or due to human led activities on the environment.

Competitive Advantage

It allows to identify all animal and plant species present in a food sample in an unprecedented way. Helps certification of origins of food and avoiding risk of presences of undesired species and origins so it's usefull for the detection of adulteration in food (chicken, beef, pork, eadable fish meat, etc.). At present no other method is as simple as rapid as the one in the present portfolio.

Other genetic tools in taxonomy use mitochondrial loci which require specific primers for different animal groups and the resulting amplicons do not vary in length (making it important to have a pure single species starting material) and are based on costly and laborious sequencing. On the contrary, the use of “universal” genetic reagents based on the nuclear gene cytoplasmic Beta actin (ACT-B) can “read through” the same DNA fragment, conserved throughout evolution and functional in the whole of the animal kingdom, simply and rapidly. This strategy also enables the identification of heterogeneous or processed biological samples, such as fish-fingers, mix-meat products, or animal remains found next to a natural reserve, without any previous knowledge or suspicion of its taxonomic classification.

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Patent Summary

U.S. Patent Classes & Classifications Covered in this listing:

Class 435: Chemistry: Molecular Biology And Microbiology

This class provides for the following subject matter when not provided for elsewhere: A process of using a microorganism or enzyme to synthesize a chemical product. A process of treating a material with a microorganism or enzyme to separate, liberate, or purify a preexisting substance. An in vitro process of measuring and testing in which: (1) A microorganism or enzyme is used to determine the presence or identity of a compound or composition in a sample; (2) A microorganism is identified by propagation; (3) An enzyme is identified by its catalytic activity; (4) The presence of microorganisms is detected; (5) A live microorganism is used in an antigen antibody test as an antigen; (6) Fixed or stabilized nonliving microorganisms, cells, or tissues are involved. A process of propagating a microorganism. A process in which the genetic structure of a microorganism or extrachromosomal genetic structure is altered. A process of organ or tissue maintenance. A process of mashing or malting. Microorganisms, per se, or the subcellular parts thereof. Enzymes, immobilized enzymes or enzyme containing compositions not otherwise provided for and the processes for purifying enzymes or forming immobilized enzymes. Compositions claimed or solely disclosed as for the propagation of microorganisms or for measuring and testing processes. Using microorganisms to destroy hazardous or toxic waste.


Class 536: Organic Compounds -- Part Of The Class 532 Series

The classes of the 532 series embrace monomeric organic carbon compounds and polymeric organic carbon compounds that are not solid synthetic resins, natural rubbers, or hydrocarbons.