Patent for Sale:

Expression system for enhancing solubility and immunogeneicity of recombinant proteins    

A simple and versatile E. coli expressing system to produce large amount of highly soluble and immunogenic recombinant proteins is introduced.

The technology is a recombinant expression system which can express a large amount of soluble recombinant proteins. The recombinant proteins are composed of three parts, a protein transduction domain (PTD), a J-domain (J) and a protein of interest (X). By way of the association between J-domain and Hsp70 chaperone, nascent PTD-J-X protein is trapped by the protein folding system efficiently to be soluble. The Hsp70::PTD-J-X complex is also involved in immune system for antigen presentation so as to enhance the antigenic presentation ability of the X protein. The PTD-J-X can penetrate through cell membrane via the PTD, therefore, the PTD-J-X can act as intracellular antigen as well.

Uniqueness:
The recombinant protein expression system is able:
1. to express very large amount of recombinant proteins in E. coli expression system,
2. to express large amount of soluble and functional recombinant proteins in E. coli expression system, and
3. to express highly immuno-presenting recombinant proteins.

Advantages:
1. A very low cost system to express very large amount of recombinant proteins.
2. A very low cost system to express large amount of soluble and functional recombinant proteins.
3. A very low cost system to express highly immuno-presenting recombinant proteins.

Maturity of technology: lab scale

1. Any protein products, such as protein drugs, growth factors, scFv, transcription factors, antimicrobial peptides, etc.
2. Animal vaccines.
3. Analytical kits.

Class 530: Chemistry: Natural Resins Or Derivatives; Peptides Or Proteins; Lignins Or Reaction Products Thereof

Subclass 324: 25 or more amino acid residues in defined sequence

Class 435: Chemistry: Molecular Biology And Microbiology

This class provides for the following subject matter when not provided for elsewhere: A process of using a microorganism or enzyme to synthesize a chemical product. A process of treating a material with a microorganism or enzyme to separate, liberate, or purify a preexisting substance. An in vitro process of measuring and testing in which: (1) A microorganism or enzyme is used to determine the presence or identity of a compound or composition in a sample; (2) A microorganism is identified by propagation; (3) An enzyme is identified by its catalytic activity; (4) The presence of microorganisms is detected; (5) A live microorganism is used in an antigen antibody test as an antigen; (6) Fixed or stabilized nonliving microorganisms, cells, or tissues are involved. A process of propagating a microorganism. A process in which the genetic structure of a microorganism or extrachromosomal genetic structure is altered. A process of organ or tissue maintenance. A process of mashing or malting. Microorganisms, per se, or the subcellular parts thereof. Enzymes, immobilized enzymes or enzyme containing compositions not otherwise provided for and the processes for purifying enzymes or forming immobilized enzymes. Compositions claimed or solely disclosed as for the propagation of microorganisms or for measuring and testing processes. Using microorganisms to destroy hazardous or toxic waste.

Subclass 69.1: Recombinant DNA technique included in method of making a protein or polypeptide
Subclass 252.3: Transformants (e.g., recombinant DNA or vector or foreign or exogenous gene containing, fused bacteria, etc.)
Subclass 320.1: VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)

Class 536: Organic Compounds -- Part Of The Class 532 Series

The classes of the 532 series embrace monomeric organic carbon compounds and polymeric organic carbon compounds that are not solid synthetic resins, natural rubbers, or hydrocarbons.

Subclass 23.1: DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)